Posters

HOT TOPIC POSTERS

I
CONFORMATIONAL STUDY OF THE ELECTRONIC INTERACTIONS AND NITRIC OXIDE RELEASE POTENTIAL OF NEW S-NITROSOTHIOL DERIVATIVES OF NON-STEROIDAL ANTI-INFLAMMATORY DRUGS (SNO-NSAID)
A.K. C. A. Reis, Universidade Federal de São Paulo  (BRAZIL)

II
EFFECTS OF DIETARY NITRATE IN PATIENTS UNDERGOING CORONARY ARTERY BYPASS
Karin E.L. Eriksson, Karolinska Institute, Stockholm  (SWEDEN)

III
PIVOTAL ROLE OF ENDOTHELIAL CELL GTP CYCLOHYDROLASE AND TETRAHYDROBIOPTERIN IN CARDIAC HYPERTROPHY IN MICE
Chuaiphichai S, University of Oxford (UK)

IV
DETERMINING THE METABOLIC FATE OF NITROGEN OXIDE SPECIES USING ISOTOPIC TRACING AND HIGH RESOLUTION MASS SPECTROMETRY
Steven J. Mullett, University of Pittsburgh (USA)

POSTERS

P-1
NITRITE AND GSNO EXERT ANTIOXIDANT EFFECT BUT ONLY GSNO ACTIVATES NRF2 PATHWAY IN VITRO
Amaral JH*1, Rizzi ES 1, Alves-Lopes R 1, Pinheiro LC 1, Tostes RC 1, Tanus-Santos JE 1
1 Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, BRAZIL

P-2
REACTIVE SULFUR SPECIES INHIBIT CA2+/ CALMODULIN DEPENDENT PROTEIN KINASE II ACTIVITY VIA SITE SPECIFIC S-POLYSULFIDATION
Shoma. Araki1, Tsuyoshi. Takata1, Yukihiro. Tsuchiya1, Yasuo. Watanabe1
1 Department of Pharmacology, Showa Pharmaceutical University, Machida, JAPAN

P-3
HUMAN ADIPOSE-DERIVED MULTIPOTENT MESENCHYMAL STROMAL CELLS LOADED WITH MICROCAPSULES AS A NEW STRATEGY FOR DRUG DELIVERY
E.N. Atochina-Vasserman1,2*, L.S. Litvinova3, V.V. Shupletsova3, O.G. Khaziakhmatova3, K.A. Yurova3 , V.V. Malashchenko3, A.S. Timin1, L. Pokrovskaya4,  E. Korotkova1, V. Popova1, D.N. Atochin1,5, G. B. Sukhorukov1,6, A. J. Gow1,7, I.A. Khlusov3,4

P-4
EFFECT OF COLD EXPOSURE ON DIETARY NITRATE METABOLISM AND BLOOD PRESSURE FOLLOWING THE ACUTE INGESTION OF NITRATE-RICH BEETROOT JUICE
Bailey SJ*, Rowland S, James LJ, O’Donnell E
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK

P-5
THE α SUBUNIT OF THE NO-RECEPTOR GUANYLYL CYCLASE IS A TRANSNITROSYLASE ACTING VIA OXIDIZED THIOREDOXIN 1 TO MODULATE CELLULAR S-NITROSATION
Wu C.2, Cui C.1,2, Alapa M.1, Shu P. 1, Liu T. 2, Crassous P. 1, Li H. 2, Beuve A*.1
1. Department of Pharmacology, Physiology and Neuroscience, Rutgers, New Jersey Medical School, Newark, NJ, USA.
2. Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, New Jersey Medical School, Newark, NJ, USA

P-6
EFFECTS OF NITRATE TREATMENT ON RENAL AND CARDIOVASCULAR DYSFUNCTION FOLLOWING ISCHEMIA-REPERFUSION OF THE KIDNEY
Zhuge Z. 1#, Zhang G. 1#, Montenegro M. 1, Lundberg J.O. 1, Weitzberg E. 1, Carlstrom M. 1*
1. Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, SWEDEN

P-7
AMP-ACTIVATED PROTEIN KINASE ACTIVATION AND NADPH OXIDASE INHIBITION BY DIETARY NITRATE PREVENTS DIET-INDUCED LIVER STEATOSIS
Cordero-Herrera I, Kozyra M, Zhuge Z, Huirong H, de Campos Cruz J, McCann Haworth S, Jahandideh A, Ingelman-Sundberg M, Weitzberg E, Lundberg JO # and Carlstrom M #*
*Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, SWEDEN

P-8
LOSS OF MYOCYTE SPECIFIC TETRAHYDROBIOPTERIN LEADS TO DILATED CARDIOMYOPATHY
Douglas G, Chu SM*, Bendall JK, Chuaiphichai S, Ricardo Carnicer, Hale A, Crabtree MJ, Channon KM
BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, UK.

P-9
IDENTIFYING SMALL MOLECULE ENHANCERS OF HEAT SHOCK PROTEIN 70 THAT LEAD TO UBIQUITINATION AND DEGRADATION OF NEURONAL NITRIC OXIDE SYNTHASE
Davis A.K.*1, Zhang H.1, Lau M.1, Chakraborty S.1, Morishima Y.1, Lieberman A.P.2, Pratt W.B.1 & Osawa Y.1
1Department of Pharmacology, University of Michigan, Ann Arbor, MI, United States of America
2Department of Pathology, University of Michigan, Ann Arbor, MI, USA

P-10
pH EFFECT ON THE PATHWAYS OF NITRIC OXIDE RELEASE FROM S-NITROSOGLUTATHIONE
de Souza GFP, Denadai JP, Picheth GF, de Oliveira MG*
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, BRAZIL

P-11
TOPICAL NITRIC OXIDE-RELEASING MESHES PROMOTE DOSE-RESPONSE DERMAL VASODILATION
Giglio LP, Alves, SDF, de Oliveira MG*
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, BRAZIL

P-12
RESTORATION OF AGE-DEPENDENT PHENOTYPES BY NITRITE REVEALS A REGULATORY ROLE FOR ENDOTHELIAL NITRIC OXIDE SYNTHASE/NITRIC OXIDE SIGNALING IN METABOLIC HOMEOSTASIS
Margarita Tenopoulou*, Paschalis-Thomas Doulias*, Kent Nakamoto, Kiara Berrios, Gabriella Zura, Chenxi Li, Michael Faust, Veronika Yakovishina, Perry Evans, Lu Tan, Michael J Bennett, Nathaniel W Snyder, William J Quinn III, Joseph A Baur, Dmitriy N Atochin, Paul L Huang and Harry Ischiropoulos
Children’s Hospital of Philadelphia Research Institute, Philadelphia, Pennsylvania, USA

P-13
HIGH SALT INDUCES HDAC1-DEPENDENT DISRUPTION OF NITRIC OXIDE SIGNALING IN THE RENAL MICROVASCULATURE
Dunaway LS*, Cook AK, Pollock DM, Hyndman KA, Inscho EW, Pollock JS
Cardio-Renal Physiology and Medicine, Division of Nephrology, University of Alabama at Birmingham, Birmingham, AL 35233 USA

P-14
FRIENDS OR FOES? PRODUCT ANALYSIS OF REACTIONS BETWEEN GSNO AND H2S: CHARACTERIZATION OF THE N- AND S- SPECIATION
Murugaeson R Kumar, Tara Clover, Abayomi D Olaitan, Christopher Becker, Touradj Solouki and Patrick J Farmer*
Department of Chemistry and Biochemistry, Baylor University, Waco TX 76798, USA

P-15
REGULATORY ROLE OF AN ISOFORM-SPECIFIC RESIDUE AT THE CALMODULIN-HEME(NO SYNTHASE) INTERFACE IN THE FMN HEME INTERDOMAIN ELECTRON TRANSFER
Jinghui Li 1 ‡, Huayu Zheng 1,2, Changjian Feng 1,2 *
1 College of Pharmacy, University of New Mexico, Albuquerque, NM 87131, USA
2 Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM 87131, USA

P-16
DIETARY NITRATE SUPRESSES PLATELET REACTIVITY IN ENDOTHELIAL NITRIC OXIDE SYNTHASE KNOCK OUT (eNOS KO) MICE
*Filomena F.1, *Parakaw T.1, Khambata R.1 & Ahluwalia A.1
1Clinical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK

P-17
PHARMACOLOGICAL INHIBITION OF FORKHEAD-BOX CLASS O TRANSCRIPTION FACTORS REDUCES NITRIC OXIDE SIGNALLING THROUGH DOWNREGULATION OF SOLUBLE GUANYLYL CYCLASE
J.C. Galley*1,2, A.C. Straub1,2
1Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, U.S.A.
2Heart, Lung, Blood, and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, U.S.A.

P-18
ROLE OF NO IN MEDIATING MACROPHAGE ACTIVATION POST OZONE EXPOSURE IN HUMAN AND ANIMAL MODELS
Laskin D1, Taylor S1, Abramova E1, Black K2, Murray A1, Francis M1, Gow A1, Kipen H2, Laskin J2
1Department of Pharmacology & Toxicology, Rutgers University, Piscataway NJ
2Environmental and Occuaptional Health Sciences Institute, Rutgers University, Piscataway NJ

P-19
THE ROLE OF cGMP/ cGKI SIGNALLING IN DIABETIC NEPHROPATHY
Harloff M.1*, Hofmann F.2, Schlossmann J.1
1 Department of Pharmacology and Toxicology, University of Regensburg, Regensburg, Germany
2 Institute of Pharmacology and Toxicology, Technical University of Munich, Munich, Germany

P-20
HYPEROXIA DECREASES TETRAHYDROBIOPTERIN IN PLASMA AND NITRIC OXIDE IN EXHALED BREATH
Hesthammer R*1,2, Dahle S2, Storesund JP2, Eide T2, Djurhuus R1, Svardal A2, Thorsen E1,2
1Norwegian Centre for Maritime and Diving Medicine, Haukeland University Hospital, Bergen, Norway
2Department of Clinical Science, University of Bergen, Norway

P-21
NO-MEDIATED HOMEOSTATIC PLASTICITY IN THE GUINEA PIG VENTRAL COCHLEAR NUCLEUS: A POTENTIAL TINNITUS GENERATION MECHANISM?
Hockley, A.,1,2 Berger, J.I.,1 Hill, S.M.D.,1 Smith, P.A.,2 Palmer, A.R.,1 Wallace, M.N.,1
1 MRC Institute of Hearing Research, University of Nottingham, Nottingham
2 School of Life Sciences, University of Nottingham, Nottingham

P-22
IDENTIFICATION OF SOLUBLE GUANYLATE CYCLASE MODULATORS FOR DISORDERS OF THE CNS
Hollas M.A.,* Ben Aissa M., Bloem L., Kim L., Lee S.H., Thatcher G.R.J.
Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, USA

P-23
DIETARY NITRATE AND NITRITE DIFFERENTIALLY ALTER OXYGEN CONSUMPTION AND  ENERGY HOMEOSTASIS IN  ZEBRAFISH (DANIO RERIO) DURING EXERCISE 
R.M. Keller1, E.R. Axton1,2,3, L.M. Beaver1,2, M.G. Jaramillo1,2, L. Truong4, R. Tanguay4, J.F. Stevens2, N.G. Hord1*
1 School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, USA
2Linus Pauling Institute, Oregon State University, Corvallis, USA
3University of California Davis Genome Center, Davis, USA
4Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, USA

P-24
TRANSLATION-COUPLED PROTEIN POLYSULFIDATION, A UNIQUE BIOSYNTHESIS PATHWAY OF CYSTEINE PERSULFIDE
Ida*1, A. Nishimura1, M. Morita1, H. Ihara2, T. Sawa3, S. Fujii1, H. Motohashi4, T. Akaike1
1Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, JAPAN
2Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, JAPAN
3Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, JAPAN
4Department of Gene Expression Regulation, Institute of Development, Aging and Cancer, Tohoku University, Sendai, JAPAN

P-25
A NOVEL MECHANISM FOR ELECTROPHILIC CYTOTOXICITY VIA IMPAIRMENT OF REACTIVE PERSULFIDE SPECIES-REGULATED REDOX SIGNALING
H Ihara*1, S Kasamatsu 1, 2, M Nishida 3, T Sawa 4, Y Kumagai 5, T Akaike 2
1 Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, JAPAN
2 Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Japa JAPAN n
3 Division of Cardiocirculatory Signaling, Okazaki Institute for Integrative Bioscience, Aichi, JAPAN
4 Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, JAPAN
5 Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, JAPAN

P-26
SEX DIFFERENCES IN RELAXANT RESPONSES OF RAT AORTAS TO CGMP GENERATORS
Ishibashi T*., Tawa M., Yamashita Y. and Masuoka T.
Department of Pharmacology, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293 JAPAN

P-27
THE CENTRAL NERVOUS SYSTEM PENETRANT SOLUBLE GUANYLATE CYCLASE STIMULATOR IWP-550 SUPPRESSED MARKERS OF NEUROINFLAMMATION IN MICE AND RATS
Juli E. Jones, Guang Liu, Susana S. Correia, Chad D. Schwartzkopf, Sarah Jacobson, Andrew Carvalho, Peter Germano, Emily Atwater, Rajesh R. Iyengar, Mark G. Currie, Christopher J. Winrow and John R. Hadcock
Ironwood Pharmaceuticals Inc., 301 Binney Street, Cambridge, USA

P-28
SODIUM NITRITE-MEDIATED CARDIOPROTECTION IN PRIMARY PERCUTANEOUS CORONARY INTERVENTION FOR ST-ELEVATION MYOCARDIAL INFARCTION: A COST-EFFECTIVENESS ANALYSIS
1,2,3Jones DA, MRCP, MSc, PhD, 3,4Whittaker P, MSc, PhD, 1,2Rathod KS, MRCP, 2Richards AJ, BSc, 2Andiapen M, 2Antoniou S, 1,2Mathur A, FRCP, PhD, 1,2Ahluwalia A, PhD.
1Centre of Clinical Pharmacology, William Harvey Research Institute, Barts & The London Medical School, Queen Mary University, London
2 Department of Cardiology, Barts Heart Centre, Barts Health NHS Trust
3 Department of Health Policy, London School of Economics and Political Science
4 Cardiovascular Research Institute & Department of Emergency Medicine, Wayne State University, Detroit, USA

P-29
MOLYBDENUM-DEPENDENT SULFITE OXIDASE REDUCES NITRITE TO NITRIC OXIDE IN VITRO AND IN VIVO
Kaczmarek A.T.*1,2, Bender D.1,2, Hagedoorn P.L.3, Schwarz G.1,2,4
1Institute of Biochemistry, Department of Chemistry, University of Cologne, Cologne, GERMANY
2Center for Molecular Medicine Cologne, University of Cologne, Cologne, GERMANY
3Applied Sciences Biotechnology, TU Delft, Delft, NETHERLANDS
4CECAD Cologne Excellence in Aging Research, Cologne, GERMANY

P-30
NITRIC OXIDE SUPPLY TO EXTRACORPOREAL CIRCULATION CIRCUIT PROTECTS KIDNEYS IN CARDIAC SURGERY: PROSPECTIVE RANDOMIZED STUDY
Kamenshchikov*1, Y. Podoksenov1,2, B. Kozlov1,2, I. Mandel3,4, Y. Svirko1,2, Y. Anfinogenova1, V. Evtushenko1,2, V. Lugovsky1, A. Nenakhova1, V. Shipulin1,2
1Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, RUSSIA
2Siberian State Medical University, Tomsk, RUSSIA
3I.M. Sechenov First Moscow State Medical University, Moscow, RUSSIA
4Research and Clinical Center for Specialized Medical Care and Medical Technologies, Federal Medico-Biological Agency, Moscow, RUSSIA

P-31
MODELING TOTAL DIETARY NITRATE INTAKE FROM FOODS, BEVERAGES, AND SUPPLEMENTS
M.C. Prater1, R.M. Keller1*, L.M. Beaver1,2, N.G. Hord1,3
1School of Biological and Population Health Sciences, Oregon State University, Corvallis, USA
2Linus Pauling Institute, Oregon State University, Corvallis, USA
3Celia Strickland Austin and G. Kenneth Austin III Professor in Public Health and Human Sciences

P-32
NITRATE AND NITRITE EXPOSURE ALTERS BEHAVIOR AND THE BRAIN METABOLOME IN ZEBRAFISH
L.M. Beaver1,2, M.G. Jaramillo1,2, E.R. Axton1,2,3, R.M. Keller1*, L. Truong4, R. Tanguay4, J.F. Stevens2, N.G. Hord1
1Celia Strickland Austin and G. Kenneth Austin III Professor in Public Health and Human Sciences, School of Biological and Population Health Sciences, Oregon State University, Corvallis, USA
2Linus Pauling Institute, Oregon State University, Corvallis, USA
3University of California Davis Genome Center, Davis, USA
4Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, USA

P-33
EFFECT OF NITRIC OXIDE-RELEASING DERIVATIVE OF INDOMETHACIN ON PREVOTELLA INTERMEDIA LIPOPOLYSACCHARIDE-INDUCED PRODUCTION OF PROINFLAMMATORY MEDIATORS IN MURINE MACROPHAGES
So-Hui Choe1, Eun-Young Choi1, Jin-Yi Hyeon1, In Soon Choi1 and Sung-Jo Kim2,3,4,*
1Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, KOREA; 2Department of Periodontology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Kor KOREA ea; 3Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Gyeongsangnam-do, Korea; 4Institute of Translational Dental Sciences, Pusan National University, Yangsan, Gyeongsangnam-do, KOREA

P-34
HOMOGENEOUS SINGLE-LABEL CGMP DETECTION PLATFORM FOR THE FUNCTIONAL STUDY OF SOLUBLE GUANYLATE CYCLASE AND PHOSPHODIESTERASE ACTIVITY
Kari Kopra,1,* Iraida Sharina,2 Emil Martin,2 and Harri Härmä1
1 Materials Chemistry and Chemical Analysis, Department of Chemistry, University of Turku, Turku, FINLAND
2 Division of Cardiology, Department of Internal Medicine, University of Texas Medical School at Houston, Houston, TX, USA

P-35
CIRCULATING NITRITE LEVELS ARE ASSOCIATED WITH BLOOD PRESSURE AND ARTERIAL STIFFNESS IN HYPERTENSION BUT NOT CARDIAC HYPERTROPHY
Lau CWZ*1, Nuredini GN1, Hamers AJP1, Duggan S1, Rathod KS1, Kapil V1, Ahluwalia A1
1William Harvey Research Institute, Centre for Clinical Pharmacology, Barts and the London School of Medicine and Dentistry, Barts NIHR Biomedical Research Centre, Queen Mary University of London, London, UK

P-36
DISRUPTION OF METHYLARGININE METABOLISM IMPAIRS VASCULAR HOMEOSTASIS DURING PREGNANCY
Georgopoulou, A.1&2, Noor, MN.1&2, Johnson, M.2 and Leiper, J.1&3*
1MRC London Institute of Medical Sciences, Imperial College London. 2Faculty of Medicine, Department of Surgery and Cancer, Imperial College London. 3Institute of Cardiovascular and Medical Sciences, University of Glasgow, Scotland, UK

 

P-37
COMPARATIVE ANALYSIS OF NITRATE-REDUCING MICROBIAL COMMUNITIES IN THE ORAL CAVITY
L’Heureux. JE*1, Ames. RM2, van der Giezen. M3, Vanhatalo. A1, Winyard. PG5, Jones. AM1.
1Sport and Health Sciences, University of Exeter, Exeter, United Kingdom; 2Wellcome Trust Centre for Biomedical Modelling and Analysis, Living Systems Institute, University of Exeter, Exeter, UK; 3Biosciences, University of Exeter, Exeter UK; 4 University of Exeter Medical School and NIHR Exeter Clinical Research Facility, Royal Devon and Exeter Hospital, Exeter, Devon, UK

P-38
RED LIGHT STIMULATES NO DEPENDENT VASODILATION IN HUMAN SUBJECTS
Fabry N 1, Marzlin N 1, Dabbouseh N 1, Keszler A 1, Lindemer B 1, Hogg N 2, Lohr N*1,3
1. Department of Medicine, Cardiovascular Medicine, Medical College of Wisconsin<
2.Department of Biophysics, Medical College of Wisconsin, USA3. Clement J Zablocki VA Medical Center, Milwaukee, USA

P-39
NITRITE-DEPENDENT NITRIC OXIDE FORMATION IN HUMANS: ROLE OF MOLYBDOENZYMES
Maia L.*, Moura J.
LAQV, REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal

P-40
HIGHER SUSCEPTIBILITY TO OXIDATION AND LOWER PROTEIN STABILITY FOR THE Α1C517T/Β1 SGC VARIANT ASSOCIATED WITH MOYAMOYA DISEASE
Iraida Sharina1, Karina Lezgiyeva2, Yekaterina Krutsenko2 and Emil Martin1 *
1 – University of Texas Health Science Center in Houston, McGovern Medical School, Department of Internal Medicine, Division of Cardiovascular Disease, Houston, USA
2– School of Science and Technology, Nazarbayev University, Astana, KAZAKHSTAN

P-41
PHARMACOLOGICAL ANALYSIS OF THE NO-SGC-CGMP PATHWAY IN LIVER FIBROSIS
J. Masferrer, S.G. Bernier, S. Jacobson, M.C. Currie, K.C. Hall Ironwood Pharmaceuticals Inc., Cambridge, USA

P-42
IMPLICATION OF SPECIFIC MUTATIONS OF THE HUMAN Xdh GENE ON SUPEROXIDE AND NITRIC OXIDE-GENERATING CAPACITY OF XANTHINE OXIDOREDUCTASE
*Massimo G.1, Khambata R.S1, Ahluwalia A.1
1William Harvey Research Institute, Barts & The London School of Medicine & Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK

P-43
BIOCHEMICAL CHARACTERIZATION OF ARABIDOPSIS THALIANA NITRATE REDUCTASE ISOFORMS 1 AND 2 IN NITRIC OXIDE SYNTHESIS
Mohn, M. A.* 1, Niks, D. 2, Hille, R. 2 and Fischer-Schrader, K. 1
1 Institute of Biochemistry, Department of Chemistry, University of Cologne, 50674 Cologne, GERMANY
2 Department of Biochemistry, University of California, Riverside, CA 92521, USA

P-44
INCREASING CONCENTRATIONS OF NITRIC OXIDE AND ACTIVATION OF SRC KINASE PROMOTE RESISTANCE TO ANOIKIS IN TUMOR CELL LINES
H.P. Monteiro*1, A. Stern2, P.E. da Costa1
1 Departament of Biochemistry, Universidade Federal de São Paulo, São Paulo, BRAZIL
2New York University School of Medicine, New York, USA

P-45
PARENTERAL L-ARGININE IMPROVES MITOCHONDRIAL FUNCTION IN CHILDREN WITH SICKLE CELL DISEASE ADMITTED FOR VASO-OCCLUSIVE PAIN EPISODES
Shiva S1, Brown LA2, Wang Y1, Dampier C2,3, Watt, A3,Tripathi V2, Bhutta A2, Kumari P2, Figueroa J2, Zmitrovich A3, and *Morris CR2,3
1University of Pittsburgh, Pittsburgh, PA, 2Emory University School of Medicine, Atlanta, GA, USA 3Children’s Healthcare of Atlanta, Atlanta, GA, USA

P-46
NITRITE IMPROVES CARDIAC FUNCTION IN RENOVASCULAR HYPERTENSION
Neto-Neves. EM1*, Pinheiro. LC1, Portella. RL1, and Tanus-Santos. JE1
1Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil

P-47
GUANYLYL CYCLASE STIMULATION MITIGATES SKELETAL AND CARDIAC MUSCLE DYSFUNCTION IN THE MDX MOUSE MODEL OF DUCHENNE MUSCULAR DYSTROPHY
Balke, J.E.1, Zhang, L.1, Xu, Y.1, Buys, ES.2, Percival J.M.1*
1Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL.
2Anesthesia Center for Critical Care Research, Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School. Boston, MA, USA

P-48
SKELETAL MUSCLE AS THE LARGEST BODY NITRATE RESERVOIR
Piknova B*, Park JW, Srihirun S, Dey S, Gilliard CN, Swanson KM, Lam KK, Cassel KS, Noguchi CT and Schechter AN
Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

P-49
HIGH SALT INDUCED ACTIVATION OF RENAL COLLECTING DUCT NOS1b PROMOTES NATRIURESIS AND BLOOD PRESSURE CONTROL
Pollock JS*, Sedaka R, Pollock DM, Hyndman KA
CardioRenal Physiology and Medicine, University of Alabama at Birmingham, Birmingham, AL 35233 USA

P-49
VIABILITY OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES LOADED WITH SYNTHETIC MICROCAPSULES IN VITRO
V Popova1*, T.A. Nevzorova2, M.Yu. Nikitina2, A.G. Daminova2, Y.V. Tarakanchikova3, D.N. Atochin1,4, E. Korotkova1, R.I. Litvinov2,5, G.B. Sukhorukov1,6, A.J. Gow1,7, E.N. Atochina-Vasserman1,2

P-50
ELEVATED NITRITE (NO2) LEVELS EXERT BENEFICIAL RENO-PROTECTIVE EFFECTS IN INDIVIDUALS UNDERGOING PERCUTANEOUS CORONARY INTERVENTION (PCI)
Rathod KS 1, Jones DA 1, Hamers AJP 1, Mathur A 1, Ahluwalia A 1
1 Queen Mary University of London, Barts and The London School of Medicine and Dentistry, London, UK

P-51
FOLIC ACID, RIBOFLAVIN, AND LPS-INDUCED NO PRODUCTION IN RAW 264.7 MURINE MACROPHAGE CELLS
Marijke Rittmann1, Brittany Friedson1, Joshua W. Miller1, Andrew Gow2*
1Nutritional Sciences, Rutgers University, New Brunswick, NJ, USA
2Ernst Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA

P-52
THE ROLE OF EXTRACELLULAR CYCLIC GMP IN HEPATOPROTECTION
Nicole M. Santerre1, Meihong Deng1, Melanie J. Scott1, Timothy R. Billiar1
All affiliated with the University of Pittsburgh, Department of General Surgery, USA

P-53
RAPID GLUTATHIONE EFFLUX UPON ATP STIMULATION AS A NOVEL REGULATORY MECHANISM FOR NLRP3 INFLAMMASOME ACTIVATION IN MACROPHAGES
Zhang T.1, Tsutsuki H.1, Ono K.1, Akaike T.2, Sawa T*1.
1. Department of Microbiology, Graduate School of Medical Science, Kumamoto University, Kumamoto, JAPAN
2. Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, JAPAN

P-54
REGULATION OF RENIN VIA cGMP/PKG
Schramm A.1,*, Schweda F.2, Sandner P.3, Hofmann F.4, Kurtz A.2, Schlossmann J.1
1 Department of Pharmacology and Toxicology, University of Regensburg, Regensburg, GERMANY
2 Institute of Physiology, University of Regensburg, Regensburg, GERMANY
3 Bayer Pharma AG, Wuppertal, GERMANY
4 Institute of Pharmacology and Toxicology, Technical University of Munich, Munich, GERMANY

P-55
THE EFFECTS OF PRO-INFLAMMATORY STIMULI ON NITRIC OXIDE PRODUCTION IN AN IN VITRO MODEL OF GLIOMA
Aalishaa A. Azam1, Jade Bailey2, Mark J. Crabtree2, Andrew Shaw*1
1School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK
2BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK

P-56
NITRATE METABOLISM IN HUMAN SKELETAL MUSCLE CELL CULTURES
Srihirun S*, Piknova B and Schechter AN.
Molecular Medicine Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA

P-57
THE β -ADRENOCEPTOR / NO SYNTHASE AXIS IS PIVOTAL TO BOTH EARLY MORTALITY RISK AND LV DYSFUNCTION IN TS
Surikow SY*1,2, Nguyen TH1,2, Stafford I2, Chapman M2, Horowitz JD1,2
1. Dept of Medicine, University of Adelaide, AUSTRALIA
2. Dept of Cardiology, The Queen Elizabeth Hospital, Adelaide, AUSTRALIA

P-58
UNDERSTANDING THE HEME REGULATORY NETWORK CONTROLLING NOX5 HEME INSERTION AND ACTIVITY
Sweeny, EA* and Stuehr DJ Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland OH 44195, USA

P-59
S-NITROSOTHIOL SIGNALLING INDUCES GLOBAL DNA HYPOMETHYLATION
C> H. Switzer*1, P. Eaton1
1School of Cardiovascular Medicine & Sciences, King’s College London, London, UK

P-60
REGULATION OF CALCIUM ION/CALMODULIN-DEPENDENT PROTEIN KINASE I BY S-POLYSULFIDATION
Tsuyoshi Takata*1, Yukihiro Tsuchiya1, Yasuo Watanabe1
1Department of Pharmacology, High Technology Research Center, Showa Pharmaceutical University, Machida, Tokyo 194-8543, JAPAN

P-61
ALTERED NO METABOLISM CAN OPPOSE LEWIS LUNG CARCINOMA CELL CONTROL OF MACROPHAGE PHENOTYPE
Taylor S1, Abramova E1, Gupta S1, Guo C1, Gow A1
1Department of Pharmacology & Toxicology, Rutgers University, Piscataway NJ, USA

P-62
SOLUBLE GUANYLATE CYCLASE STIMULATOR IW-1701 ATTENUATES ACTIVATION OF ENDOTHELIAL CELLS AND LEUKOCYTES IN MOUSE MODEL OF TNFa-INDUCED INFLAMMATION.
B. Tchernychev*1, S. Feil2, R. Feil2, J.R. Hadcock1, G.T. Milne1, M.G. Currie1, R.M. Graul1, J. Masferrer1
1Ironwood Pharmaceuticals Inc., Cambridge, USA
2Interfaculty Institute of Biochemistry, University of Tübingen, Germany

P-63
THE LABILE IRON POOL (LIP) CAN NO LONGER BE CONSIDERED SOLELY A PRO-OXIDATIVE CELLULAR IRON SOURCE
Fernando Cruvinel Damasceno1, André Luis Condeles1, Angélica Kodama Bueno Lopes1, Rômulo Rodrigues Facci1, Edlaine Linares2, Daniela Ramos Truzzi2, Ohara Augusto2 and José Carlos Toledo Junior1,*
1. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, CEP, 14040-901, BRAZIL
2. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, CEP 05508-000, BRAZIL

P-64
NEURONAL NOS EXPRESSION PROMOTES ACETYLCHOLINE RECEPTOR SIGNALING ENHANCEMENT
Yukihiro Tsuchiya*1, Tsuyoshi Takata1, Hideshi Ihara2, Yasuo Watanabe1
1 Department of Pharmacology, Showa Pharmaceutical University, Tokyo, JAPAN
2 Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, JAPAN

P-66
PROTECTION OF MICE AGAINST LETHAL ENDOTOXIN SHOCK BY NOVEL PERSULFIDE DONORS BASED ON N-ACETYL-L-CYSTEINE
Tsutsuki H.*1, Zhang T.1, Ono K.1, Ihara H.2, Akaike T.3, Sawa T.1
1Department of Microbiology, Graduate School of Medical Science, Kumamoto University, Kumamoto, JAPAN
2Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, JAPAN
3Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, JAPAN

P-67
NITRITE BIOACTIVATION BY RED BLOOD CELLS POTENTIATED BY FAR RED LIGHT; APPLICATIONS IN THROMBOSIS
N. Wajih1*, S. Basu1, K.B. Ucer1, A. Perlegas1, K. Simms2, E. Rahbar2, M.Guthold1, L.M. Smith3, D.B. Kim-Shapiro1
1. Department of Physics, Wake Forest University, Winston-Salem, USA
2. Department of Biomedical Engineering, Wake Forest University School of Medicine, Winston-Salem, USA
3. Department of Emergency Medicine, Wake Forest University School of Medicine, Winston-Salem, USA

P-68
REGULATION OF CYSTATHIONINE γ -LYASE BY CYSTEINE HYDROPERSULFIDE
Yasuo Watanabe*1, Shoma Araki1, Tsuyoshi Takata1, Yukihiro Tsuchiya1, Takaaki Akaike2
1Department of Pharmacology, High Technology Research Center, Showa Pharmaceutical University, Machida, Tokyo 194-8543, JAPAN
2 Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, JAPAN

P-69
ROLE OF SPAK IN NO PRODUCTION AND VASCULAR HYPOREACTIVITY IN ENDOTOXAEMIC MICE
Chih-Chin Shih1, Shiu-Jen Chen2, Lin-Pin Hsu1, Mei-Hui Liao1, Sung-Sen Yang3, Shung-Tai Ho4, Chin-Chen Wu1,*
1Department of Pharmacology, 3Department of Nephrology, Tri-Service General Hospital, National Defense Medical Center, TAIPEI; 2Department of Long-Term Care, College of Nursing and Health, Kang-Ning University, Taipei; 4Department of Anesthesiology, TAIPEI Veterans General Hospital, Taipei; 5Department of Anesthesiology, Tungs’ Taichung MetroHarbor Hospital, Taichung; TAIWAN/ROC

P-70
iNOS/NO/TACE REGULATED NOTCH SIGNALING PREVENTS CD4+ T CELL APOPTOSIS DURING ENDOTOXEMIA.
C> Yang*1,2, M. Deng2, M. Scott2, T. R. Billiar2*
Tsinghua University School of Medicine, P.R. CHINA
Department of Surgery, University of Pittsburgh School of Medicine, USA